SeaD Consulting understands the importance of food safety in our industry. Explore our advanced food safety technology for various levels of industry. Contact us to discuss how we can tailor solutions to meet the specific needs of your business.
At the core of our pathogen detection suite is Digital PCR (dPCR) technology—a more robust and precise alternative to conventional qPCR. We specialize in providing highly reproducible results for difficult samples, ensuring your products meet the highest safety standards.
Why choose our dPCR assays?
No DNA Extraction Required: Skip complex prep; our technology is built to withstand common PCR inhibitors.
Direct Results: Achieve absolute quantification without the hassle of standard curves.
Enhanced Precision: Detect multiple gene targets within a cell with unparalleled sensitivity and reproducibility.
Ensuring Regulatory Compliance with Unmatched Accuracy Enterohemorrhagic Escherichia coli (EHEC), or Shiga toxin-producing E. coli (STEC), represents a critical safety risk in the food supply chain. With the USDA maintaining a zero-tolerance policy for these strains in red meat, the industry requires a reliable diagnostic assay.
The Challenge: False Positives Conventional real-time PCR assays often struggle with high false-positive rates. These inaccuracies lead to unnecessary product holds, wasted resources, and significant financial loss for meat producers.
Our Innovation: Single-Cell Logic Our dPCR assay eliminates the "background noise" from other Enterobacteriaceae and bacteriophage cells that plagues traditional methods. By analyzing bacterial cells directly from the enrichment, our technology verifies the presence of the two essential virulence genes (stx and eae) within the same cell.
Rapid Results: Go from sample to quantified result in just 11 hours.
Precision: Eliminates false-positive signals from free-floating DNA or non-pathogenic bacteria.
Efficiency: A simplified workflow designed for high-throughput testing of food enrichments.
Reliability: Provides the definitive "yes/no" answer required for high-stakes regulatory environments.
Our Digital PCR (dPCR) technology offers a robust solution for the absolute quantification of Salmonella in meat and poultry matrices. By moving beyond traditional qualitative testing, we enable a more granular understanding of microbial loads in the production chain.
Beyond Presence/Absence, most commercial methods only indicate if Salmonella is present. Our assay provides absolute quantification, allowing producers to identify and isolate heavily contaminated samples that pose the highest risk for large-scale outbreaks.
Rapid Results: Go from sample to quantified result in just 9 hours.
Streamlined Workflow: Features a short 6-hour enrichment followed by direct cell analysis—no complex DNA extraction required.
Validated Matrices: Optimized for high reliability in ground beef and chicken breast samples.
Actionable Insights: Empower your quality control team with exact pathogen loads to make informed "release or hold" decisions.